Human Endophilin B1/Bif-1 Antibody

Name: Human Endophilin B1/Bif-1 Antibody
Brand: R&D Systems
SKU: AF7456

R&D Systems, part of Bio-Techne | Catalog # AF7456

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Endophilin B1/Bif-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Endophilin B1/Bif-1
Ala33-Asn189
Accession # Q9Y371

Specificity

Detects human Endophilin B1/Bif-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SH3GLB2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Endophilin B1/Bif-1 Antibody

Detection of Human Endophilin B1/Bif‑1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Saos-2 human osteosarcoma cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Endophilin B1/Bif-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7456) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Endophilin B1/Bif-1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Endophilin B1/Bif‑1 in Jurkat Human Cell Line.

Endophilin B1/Bif-1 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human Endophilin B1/Bif-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7456) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Endophilin B1/Bif‑1 in Human Pancreatic Cancer Tissue.

Endophilin B1/Bif-1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Sheep Anti-Human Endophilin B1/Bif-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7456) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Endophilin B1/Bif-1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Saos‑2 human osteosarcoma cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endophilin B1/Bif-1

Endophilin-B1, also known as SH3GLB1 (Src-Homology 3 Domain-containing GRB2-like protein B1) and BIF-1, is a 40 kDa protein that belongs to the endophilin family of molecules. It is a cytoplasmic and Golgi membrane protein that is expressed in neurons, striated (skeletal and cardiac) muscle cells, and placenta. Endophilin-B1 plays a role in cell homeostasis. In neurons, Endophilin-B1 is phosphorylated by Cdk5, inducing homodimerization and autophagosome formation. In addition, it appears to play a role in the maintenance of mitochondrial integrity. Endophilin-B1 appears to cycle on-and-off the outer mitochondrial membrane (OMM), contributing to OMM integrity. And after the initiation of endocytosis, it also directs EEA1+ TrkA-containing endosomes back into the cell membrane for reuse. Human Endophilin‑B1 is 365 amino acids (aa) in length. It possesses an N-terminal lipid-binding BAR (Bin/Amphiphysin/Rvs) domain (aa 27-261) that contains an internal coiled-coil region, and a C-terminal SH3 domain that binds Pro-rich sequences on potential ligands (309-363). There are at least three potential splice variants for Endophilin-B1. Two are 42 and 43 kDa in size, and possess a 21 and 29 aa insertion after Ser190, respectively. A third potentially utilizes an alternative start site at Met101. Over aa 33-189, human Endophilin-B1 shares 98% aa sequence identity with mouse Endophilin-B1.

Long Name

SH3-domain GRB2-like Endophilin B1/Bax-interacting Factor 1

Alternate Names

Bif-1, Bif1, SH3GLB1

Entrez Gene IDs

51100 (Human); 54673 (Mouse); 292156 (Rat)

Gene Symbol

SH3GLB1

UniProt

Q9Y371

Additional Endophilin B1/Bif-1 Products

Product Documents for Human Endophilin B1/Bif-1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Endophilin B1/Bif-1 Antibody

For research use only

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