Human Endothelin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB34402
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1065402
Product Summary for Human Endothelin-1 Antibody
Immunogen
Synthetic peptide containing EDN1.
Accession # P05305
Accession # P05305
Specificity
Detects human Endothelin-1 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Endothelin-1 Antibody
Detection of Endothelin-1 in Huvec (positive) and Jurkat (negative) cells.
Endothelin-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells (positive) and absent in Jurkat human acute T cell leukemia cells (negative) using Mouse Anti-Human Endothelin-1 Monoclonal Antibody (Catalog # MAB34402) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Endothelin-1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells (positive) and Jurkat human acute T cell leukemia cells (negative)
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells (positive) and Jurkat human acute T cell leukemia cells (negative)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endothelin-1
Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).
Alternate Names
ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME
Gene Symbol
EDN1
UniProt
Additional Endothelin-1 Products
Product Documents for Human Endothelin-1 Antibody
Product Specific Notices for Human Endothelin-1 Antibody
For research use only
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