Human Enolase 2/Neuron-specific Enolase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB51691
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 972458
Product Specifications
Immunogen
E. coli-derived recombinant human Enolase 2/Neuron‑specific Enolase
Met1-Leu434
Accession # P09104
Met1-Leu434
Accession # P09104
Specificity
Detects human Enolase 2/Neuron‑specific Enolase in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Enolase 2/Neuron-specific Enolase Antibody
Detection of Human, Mouse, and Rat Enolase 2/Neuron‑specific Enolase by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SH-SY5Y human neuroblastoma cell line, human brain tissue (cerebellum), mouse brain tissue (cerebellum), and rat brain tissue (cerebellum). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Enolase 2/Neuron-specific Enolase Monoclonal Antibody (Catalog # MAB51691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Enolase 2/Neuron-specific Enolase at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Enolase 2/Neuron‑specific Enolase in SH‑SY5Y Human Cell Line.
Enolase 2/Neuron-specific Enolase was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Enolase 2/Neuron-specific Enolase Monoclonal Antibody (Catalog # MAB51691) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Enolase 2/Neuron-specific Enolase Antibody
Application
Recommended Usage
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, SH-SY5Y human neuroblastoma cell line, human brain tissue (cerebellum), mouse brain tissue (cerebellum), and rat brain tissue (cerebellum)
Sample: Jurkat human acute T cell leukemia cell line, SH-SY5Y human neuroblastoma cell line, human brain tissue (cerebellum), mouse brain tissue (cerebellum), and rat brain tissue (cerebellum)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Enolase 2/Neuron-specific Enolase
Alternate Names
ENO2, gamma-Enolase, Neuronspecific Enolase, NSE
Gene Symbol
ENO2
UniProt
Additional Enolase 2/Neuron-specific Enolase Products
Product Documents for Human Enolase 2/Neuron-specific Enolase Antibody
Product Specific Notices for Human Enolase 2/Neuron-specific Enolase Antibody
For research use only
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