Human/Equine Cytochrome c Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB898
Key Product Details
Species Reactivity
Validated:
Human, Equine
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 2B5F8
Product Specifications
Immunogen
Equine Cytochrome c
Specificity
Detects native human Cytochrome c but not mouse Cytochrome c.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Equine Cytochrome c Antibody
Immunoprecipitation of Human/Mouse Cytochrome c.
Cytochrome c was immunoprecipitated from lysates (5 x 106cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) for 1 hour at 4 ºC. Cytochrome c-antibody complex-es were absorbed using Protein A Immunoprecipitin (Life Tech-nologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.
Cytochrome c in Human PBMCs.
Cytochrome c was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA using Human/Equine Cytochrome c Monoclonal Antibody (Catalog # MAB898) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Equine Cytochrome c Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with ionomycin and PMA
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (basal ganglia)
Sample: Immersion fixed paraffin-embedded sections of human brain (basal ganglia)
Immunoprecipitation
1 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line and CTLL-2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Sample: Jurkat human acute T cell leukemia cell line and CTLL-2 mouse cytotoxic T cell line, see our available Western blot detection antibodies
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytochrome c
Additional Cytochrome c Products
Product Documents for Human/Equine Cytochrome c Antibody
Product Specific Notices for Human/Equine Cytochrome c Antibody
For research use only
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