Human Erythropoietin R MAb (Clone 713210)
R&D Systems, part of Bio-Techne | Catalog # MAB3073
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 713210
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Ala25-Pro250
Accession # P19235
Ala25-Pro250
Accession # P19235
Specificity
Detects human Erythropoietin R in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Erythropoietin R MAb (Clone 713210)
Proliferation Induced by Erythropoietin and Neutralization by Human Erythropoietin R Antibody.
Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND50 is typically 0.4-2.4 µg/mL.Applications for Human Erythropoietin R MAb (Clone 713210)
Application
Recommended Usage
Neutralization
Measured
by its ability to neutralize Erythropoietin-induced proliferation in the
TF‑1 human erythroleukemic cell line. The Neutralization Dose
(ND50) is typically 0.4-2.4 µg/mL in the
presence of 0.278 units/mL Recombinant Human Erythropoietin (Tissue
Culture Grade).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin R
Long Name
Erythropoietin Receptor
Alternate Names
EpoR
Gene Symbol
EPOR
UniProt
Additional Erythropoietin R Products
Product Documents for Human Erythropoietin R MAb (Clone 713210)
Product Specific Notices for Human Erythropoietin R MAb (Clone 713210)
For research use only
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