Human Exosome Component 9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11616
Key Product Details
| Species Reactivity: | Human |
| Immunogen: |
Synthetic Peptide Accession # Q06265 |
| Applications: | Immunocytochemistry/ Immunofluorescence, Immunohistochemistry |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG1 Clone # 1090209 |
| Specificity: |
Detects a synthetic peptide specific for hEXOSC9 around amino acid 300 in Direct ELISA. |
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ImmunohistochemistryDetection of Exosome Component 9 in Human Colon Cancer. Exosome Component 9 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human Exosome Component 9 Monoclonal Antibody (Catalog # MAB11616) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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ImmunocytochemistryDetection of Exosome Component 9 in U251‑MG Human Cell Line. Exosome Component 9 was detected in immersion fixed U251‑MG human malignant glioblastoma cell line using Mouse Anti-Human Exosome Component 9 Monoclonal Antibody (Catalog # MAB11616) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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