F-box protein 15/FBXO15 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Oct-3/4 was detected in cell nuclei using Goat Anti-Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) and stained using NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of F-box protein 15 was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of FBXO15 in BG01V Human Stem Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human F-box protein 15/FBXO15 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: BG01V human embryonic stem cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: F-box protein 15/FBXO15

FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 (isoform 2 of accession Q8NCQ5) is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site (isoform 1 of accession Q8NCQ5), and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.

Alternate Names

Fbox protein 15, FBX15, FBXO15

Entrez Gene IDs

201456 (Human); 361354 (Rat)

Gene Symbol

FBXO15

UniProt

Q8NCQ5-2

Additional F-box protein 15/FBXO15 Products

Product Documents for Human F-box protein 15/FBXO15 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human F-box protein 15/FBXO15 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human F-box protein 15/FBXO15 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6035

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

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Product Summary for Human F-box protein 15/FBXO15 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human F-box protein 15/FBXO15 Antibody

F‑box protein 15/FBXO15 in BG01V Human Embryonic Stem Cells.