Human FABP5/E-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3077
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Macaca fuscata (Japanese Macaque)
Applications
Validated:
Immunocytochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FABP5/E‑FABP
Ala2-Glu135
Accession # Q01469
Ala2-Glu135
Accession # Q01469
Specificity
Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) FABP5 is observed and less than 5% cross-reactivity with recombinant human FABP1, -2, -3, -4, -6, -7, and rmFABP9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human FABP5/E-FABP Antibody
Detection of Human FABP5/E‑FABP by Western Blot.
Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP5/E‑FABP in HUVEC Human Umbilical Vein Endothelial Cells.
FABP5/E-FABP was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human FABP5/E‑FABP by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue and human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E-FABP at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human FABP5/E-FABP Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Knockout Validated
FABP5/E‑FABP
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
FABP5/E‑FABP knockout HeLa cell line.
Simple Western
10-12.5 µg/mL
Sample: Human heart tissue, human placenta tissue, HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Human heart tissue, human placenta tissue, HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: Human heart tissue, human brain (cerebellum) tissue, and human placenta tissue
Sample: Human heart tissue, human brain (cerebellum) tissue, and human placenta tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP5/E-FABP
Long Name
Fatty Acid-Binding Protein 5
Alternate Names
E-FABP, EFABP
Gene Symbol
FABP5
UniProt
Additional FABP5/E-FABP Products
Product Documents for Human FABP5/E-FABP Antibody
Product Specific Notices for Human FABP5/E-FABP Antibody
For research use only
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