Human Fc gamma RIII (CD16) Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2546B

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Immunoprecipitation

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # 245536

View all Fc gamma RIII (CD16) Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
Thr20-Gln208
Accession # O75015

Specificity

Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Fc gamma RIII (CD16) Biotinylated Antibody

Detection of Fc gamma RIII (CD16) antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Fc gamma RIII (CD16) Biotinylated Monoclonal Antibody (Catalog # FAB2546B) or (B) Mouse IgG2ABiotinylated Isotype Control (IC003B) followed by Streptavidin-Phycoerythrin (F0040). View our protocol for Staining Membrane-associated Proteins.

Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R) and either (A) Mouse Anti-Human Fc gamma RIII (CD16) Biotinylated Monoclonal Antibody (Catalog # FAB2546B) or (B) Mouse IgG2ABiotinylated Isotype Control (IC003B) followed by Streptavidin-Phycoerythrin (F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Fc gamma RIII (CD16) Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

CD16 (also known as FcgRIII) is a CD designation that, in human, encompasses two related, but distinct Ig superfamily genes; CD16a and CD16b. Mature human CD16a is a 45-75 kDa type I transmembrane glycoprotein that is 238 amino acids (aa) in length. It contains a 192 aa extracellular region that possesses two Ig-like domains plus glycosylation that is critical to its function, and ends with a short 25 aa cytoplasmic domain. On the cell surface, CD16a complexes with FceRI and CD3 zeta. CD16a shows a restricted expression pattern, being found on immature thymocytes, placental trophoblast cells, CD56dim and cytokine-activated CD56+ NK cells, slan (sulfated NAcLac)-expressing dendritic cells, and CX3CR1+ CD14+/- "patrolling" monocytes. Mature CD16b, by contrast, is slightly smaller that CD16a, but still shows the same 50-70 kSDa electrophoretic pattern in SDS-PAGE. It is a GPI-linked glycoprotein that is 184 aa in length. On the cell surface, CD16b is monomeric. The standard allele (= NA2) is encoded by the SwissProt Accession # used for this product (see above). The two variant alleles are NA1, which shows four aa substitutions, and SH, which contains only one aa substitution relative to the NA2 sequence. CD16b expression appears to be limited to neutrophils. CD16b is known to be solubilized, and the soluble form is believed to arise from the action of ADAM-17 rather than a phospholipase. Over aa 20-208, human CD16a and CD16b share 97% aa sequence identity. Over the same aa range, human CD16b and mouse CD16 share only 47% aa sequence identity.

Long Name

Fc gamma Receptor III

Alternate Names

FcgRIII

Entrez Gene IDs

14131 (Mouse); 102140945 (Cynomolgus Monkey)

Gene Symbol

FCGR3

UniProt

O75015

Additional Fc gamma RIII (CD16) Products

Product Documents for Human Fc gamma RIII (CD16) Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Fc gamma RIII (CD16) Biotinylated Antibody

For research use only

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