Human FCRL6/FcRH6 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6284

R&D Systems, part of Bio-Techne
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AF6284
AF6284-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all FCRL6/FcRH6 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FCRL6/FcRH6
Lys16-Leu312
Accession # Q6DN72

Specificity

Detects human FCRL6/FcRH6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FcRH1, rhFcRH2, and rhFcRH4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FCRL6/FcRH6 Antibody

Detection of Human FCRL6/FcRH6 antibody by Western Blot.

Detection of Human FCRL6/FcRH6 by Western Blot.

Western blot shows lysates of human tonsil tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FCRL6/FcRH6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6284) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRL6/FcRH6 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human FCRL6/FcRH6 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human tonsil tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL6/FcRH6

FCRL6 (Fc receptor-like protein 6; also FcRH6 and IFGP6) is a 66 kDa member of the Ig Superfamily. It is found on CD56dim CD16+ NK cells, CD56+ CD3+ NKT cells, gd T cells, and effector plus effector-memory CD8+ T cells. FCRL6 is not a receptor for immunoglobulin. It does, however, bind to isoforms of HLA-DR that are expressed on APCs. Given that FCRL6 contains a cytoplasmic ITIM, FCRL6 may function as an inhibitory receptor for MHC Class II.  Mature human FCRL6 is a 415 amino acid (aa) type I transmembrane protein. It contains a 288 aa extracellular region (aa 20-307) that shows three C2-type Ig-like domains (aa 20-293), and a 106 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 105-200 accompanied by a 13 aa substitution for aa 394-434; a second possesses a 15 aa substitution for aa 383-434; and a third shows an alternative start site seven aa upstream of the standard site accompanied by a 13 aa substitution for aa 394-434. Over aa 16-312, human FCRL6 shares only 33% aa identity with the mouse counterpart to FCRL6.

Long Name

Fc Receptor-like 6

Alternate Names

FcRH6, IFGP6

Entrez Gene IDs

343413 (Human); 677296 (Mouse)

Gene Symbol

FCRL6

UniProt

Q6DN72 (Human)

Additional FCRL6/FcRH6 Products

Product Documents for Human FCRL6/FcRH6 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FCRL6/FcRH6 Antibody

For research use only

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