Human FGFR3 (IIIb) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1474
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 136315
Product Specifications
Immunogen
Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)
Specificity
Detects human
FGF R3 (IIIb) in direct ELISAs. In direct ELISAs, approximately 25‑50%
cross-reactivity with recombinant mouse FGF R3 (IIIb) is
observed and 12% with recombinant human (rh) FGF R4 is
observed and no cross-reactivity with (IIIc) isoforms of rhFGF R3 or any
isoforms of rhFGF R1 or rhFGF R2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human FGFR3 (IIIb) Antibody
FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R3 Antibody.
Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND50 is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL).Applications for Human FGFR3 (IIIb) Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R-3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 300 ng/mL Recombinant Human FGF R3 (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR3
Long Name
Fibroblast Growth Factor Receptor 3
Alternate Names
CD333, CEK, FGF R3, JTK4
Gene Symbol
FGFR3
Additional FGFR3 Products
Product Documents for Human FGFR3 (IIIb) Antibody
Product Specific Notices for Human FGFR3 (IIIb) Antibody
For research use only
Loading...
Loading...
Loading...