Human FGL2 Antibody

Detection of Human FGL2 by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FGL2 Monoclonal Antibody (Catalog # MAB5974) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FGL2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FGL2 in WI‑38 Human Cell Line.

FGL2 was detected in immersion fixed WI-38 human fetal lung fibroblast cell line using Mouse Anti-Human FGL2 Monoclonal Antibody (Catalog # MAB5974) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of FGL2 in Human Cerebellum.

FGL2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Mouse Anti-Human FGL2 Monoclonal Antibody (Catalog # MAB5974) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje Neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.