Human Fgr Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB32071
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 349331
Product Specifications
Immunogen
E. coli-derived recombinant human Fgr
Met1-Phe82
Accession # P09769
Met1-Phe82
Accession # P09769
Specificity
Detects human Fgr in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Fgr Antibody
Fgr in HepG2 Human Cell Line.
Fgr was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Fgr Monoclonal Antibody (Catalog # MAB32071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Fgr Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Daudi human Burkitt's lymphoma cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed Daudi human Burkitt's lymphoma cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fgr
Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of nonreceptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.
Long Name
Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog
Alternate Names
Src2
Gene Symbol
FGR
UniProt
Additional Fgr Products
Product Documents for Human Fgr Antibody
Product Specific Notices for Human Fgr Antibody
For research use only
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