Human FOLR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5697
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 583121
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human FOLR2
Gln22-His228
Accession # P14207
Gln22-His228
Accession # P14207
Specificity
Detects human FOLR2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human FOLR1, 3, 4, or recombinant mouse FOLR2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FOLR2 Antibody
FOLR2 in Human Neutrophils.
FOLR2 was detected in immersion fixed human neutrophils using Mouse Anti-Human FOLR2 Monoclonal Antibody (Catalog # MAB5697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human FOLR2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human neutrophils
Sample: Immersion fixed human neutrophils
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR2
Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa glycoprotein that is anchored to the membrane via glycosyl phosphatidylinositol. It mediates the cellular uptake of dietary folates that are required for key steps in amino acid metabolism. FOLR2 is predominantly expressed in placenta, myeloid cells, and some CD34+ hematopoietic progenitor cells. It is upregulated on some cancer cells and at sites of chronic inflammation. Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2.
Long Name
Folate Receptor 2
Alternate Names
beta-HFR, FR-beta, FR-P3
Gene Symbol
FOLR2
UniProt
Additional FOLR2 Products
Product Documents for Human FOLR2 Antibody
Product Specific Notices for Human FOLR2 Antibody
For research use only
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