Human FoxO1/FKHR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5939
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 597554
Product Specifications
Immunogen
E. coli- derived recombinant human FoxO1/FKHR
Ala353-Gly655
Accession # Q12778
Ala353-Gly655
Accession # Q12778
Specificity
Detects human FoxO1/FKHR in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxA1 (aa 253-472), B1 (aa 191-324), C1 (aa 208-322), D3 (aa 1-140), F1 (aa 129-354), G1 (aa 283-489), J1 (aa 301-421), J3 (aa 375-552), K1 (aa 493-670), M1 (aa 642-763), O3A (aa 372-673), P1 (aa 231-420), P2 (aa 269-460), P3 (aa 179-279), or P4 (aa 229-410) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FoxO1/FKHR Antibody
FoxO1/FKHR in LNCaP Human Cell Line.
FoxO1/FKHR was detected in immersion fixed LNCaP human prostate cancer cell line using Human FoxO1/FKHR Monoclonal Antibody (Catalog # MAB5939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of FoxO1/FKHR in Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT-4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells.
FoxO1/FKHR was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT-4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells using Mouse Anti-Human FoxO1/FKHR Monoclonal Antibody (Catalog # MAB5939) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human FoxO1/FKHR Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line, Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells
Sample: Immersion fixed LNCaP human prostate cancer cell line, Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxO1/FKHR
Long Name
Forkhead Box O1
Alternate Names
FKH1, FKHR
Gene Symbol
FOXO1
UniProt
Additional FoxO1/FKHR Products
Product Documents for Human FoxO1/FKHR Antibody
Product Specific Notices for Human FoxO1/FKHR Antibody
For research use only
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