Human Fucosyltransferase 8/FUT8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5768
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8
Asp32-Lys575
Accession # Q9BYC5
Asp32-Lys575
Accession # Q9BYC5
Specificity
Detects human Fucosyltransferase 8/FUT8 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Fucosyltransferase 8/FUT8 Antibody
Fucosyltransferase 8/FUT8 in Human Colon.
Fucosyltransferase 8/FUT8 was detected in paraffin-embedded sections of human colon using Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Fucosyltransferase 8/FUT8 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 60 - 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Fucosyltransferase 8/FUT8 by Simple WesternTM.
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 64 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Fucosyltransferase 8/FUT8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human colon subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 8/FUT8, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 8/FUT8, see our available Western blot detection antibodies
Simple Western
10 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line
Sample: COLO 205 human colorectal adenocarcinoma cell line
Western Blot
1 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line
Sample: COLO 205 human colorectal adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fucosyltransferase 8/FUT8
Alternate Names
alpha 1,6 Fucosyltransferase, alpha1-6FucT, FUT8
Gene Symbol
FUT8
UniProt
Additional Fucosyltransferase 8/FUT8 Products
Product Documents for Human Fucosyltransferase 8/FUT8 Antibody
Product Specific Notices for Human Fucosyltransferase 8/FUT8 Antibody
For research use only
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