Human GADD153 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7224

R&D Systems, part of Bio-Techne
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MAB7224
MAB7224-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 671708

View all GADD153 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GADD153
Met1-Ala169
Accession # P35638

Specificity

Detects human GADD153 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GADD153 Antibody

GADD153 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

GADD153 in Jurkat Human Cell Line.

GADD153 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with PMA and hydrogen peroxide using Mouse Anti-Human GADD153 Monoclonal Antibody (Catalog # MAB7224) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human GADD153 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line stimulated with PMA and hydrogen peroxide

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GADD153

GADD153, also known as CCAAT-enhancer binding protein zeta (C/EBPz), DDIT, and CHOP, is a ubiquitous 29 kDa basic-leucine zipper transcription factor. GADD153 heterodimerization with other C/EBP factors inhibits their activity and alters their DNA sequence recognition. GADD153 inhibits Wnt signaling by dimerizing with the transcription factors TCF-3 and TCF-4. It is upregulated under conditions of cellular stress including DNA damage, oxidative damage, nutrient depletion, ER stress, and mechanical stress, whereupon it promotes the apoptotic response. The transcriptional activity of GADD153 is enhanced by phosphorylation at Ser 78 and Ser 81. Human GADD153 shares 88% and 90% amino acid sequence identity with mouse and rat GADD153, respectively.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Alternate Names

CEBP zeta, CHOP, CHOP10, DDIT3

Entrez Gene IDs

1649 (Human); 13198 (Mouse); 29467 (Rat)

Gene Symbol

DDIT3

UniProt

P35638

Additional GADD153 Products

Product Documents for Human GADD153 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GADD153 Antibody

For research use only

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