Human GATA-6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1700
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 222228
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # CAA64997
Met1-Ala449
Accession # CAA64997
Specificity
Detects human GATA-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) GATA-1 or rhGATA-2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GATA-6 Antibody
GATA‑6 in HCT‑116 Human Cell Line.
GATA-6 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GATA‑6 in HT‑29 Human Cell Line.
GATA-6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GATA-6 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed KATO‑III human gastric carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and HT-29 human colon adenocarcinoma cell line
Sample: Immersion fixed KATO‑III human gastric carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and HT-29 human colon adenocarcinoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human GATA-6
Sample: Recombinant Human GATA-6
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-6
References
- Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.
Alternate Names
GATA6
Entrez Gene IDs
2627 (Human)
Gene Symbol
GATA6
UniProt
Additional GATA-6 Products
Product Documents for Human GATA-6 Antibody
Product Specific Notices for Human GATA-6 Antibody
For research use only
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