Human GGT5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7209
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GGT5
Ser30-Tyr586
Accession # P36269
Ser30-Tyr586
Accession # P36269
Specificity
Detects human GGT5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GGT5 Antibody
Detection of Human GGT5 by Western Blot.
Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GGT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GGT5 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human GGT5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human kidney (medulla) tissue
Sample: Human kidney (medulla) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GGT5
GGT5 (Gamma GlutamylTranspeptidase 5; also GGL, GGT-rel and gamma-leukotrienease) is a 74-77 kDa member of the gamma-glutamyltransferase family of enzymes. It is suggested that multiple cell types express GGT5, including neurons, lymphocytes, myocardium and bronchial epithelium. It is also expressed on the surface of endothelial cells, and serves to both metabolize GSH to CysGly, and convert LTC4 to LTD4, an action that appears necessary for targeted inflammatory cell migration. Human GGT5 is a 586 amino acid (aa) type II transmembrane (TM) glycoprotein. It undergoes processing to generate a disulfide-linked heterodimer composed of a 56-58 kDa membrane-embedded heavy chain (aa 1-387, with the TM segment lying between aa 9-29) and a 20-21 kDa light chain (aa 388-586). There is one potential splice variant that shows a deletion of aa 112-133. Over aa 30-586, human GGT5 shares 78% aa identity with mouse GGT5.
Long Name
gamma-Glutamyltransferase 5
Alternate Names
GGT-rel, GGTLA1
Gene Symbol
GGT5
UniProt
Additional GGT5 Products
Product Documents for Human GGT5 Antibody
Product Specific Notices for Human GGT5 Antibody
For research use only
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