Human GLI-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3526
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), Proximity Ligation Assay
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GLI-2
Ala2-Glu91
Accession # BAA25668
Ala2-Glu91
Accession # BAA25668
Specificity
Detects human GLI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GLI-2 Antibody
Detection of GLI‑2-regulated Genes by Chromatin Immunoprecipitation.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3526) or control antibody (Catalog # 5-001-A) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody (Catalog # BAF016).Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR.Applications for Human GLI-2 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, bcl-2 promoter detected by standard PCR
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, bcl-2 promoter detected by standard PCR
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin, basal cell carcinoma, and prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human skin, basal cell carcinoma, and prostate cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human GLI-2
Sample: Recombinant Human GLI-2
Reviewed Applications
Read 3 reviews rated 3.7 using AF3526 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-2
GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology.
Long Name
GLI-Kruppel family member GLI2
Alternate Names
GLI2, THP2
Gene Symbol
GLI2
UniProt
Additional GLI-2 Products
Product Documents for Human GLI-2 Antibody
Product Specific Notices for Human GLI-2 Antibody
For research use only
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