Human GLI-2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3526
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GLI-2
Ala2-Glu91
Accession # BAA03568
Ala2-Glu91
Accession # BAA03568
Specificity
Detects human GLI-2 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 is observed and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GLI-2 Biotinylated Antibody
GLI‑2 in MDA‑MB‑231 Human Cell Line.
GLI-2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Sheep Anti-Human GLI-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3526) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GLI-2 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human GLI-2
Sample: Recombinant Human GLI-2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-2
GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology.
Long Name
GLI-Kruppel family member GLI2
Alternate Names
GLI2, THP2
Gene Symbol
GLI2
UniProt
Additional GLI-2 Products
Product Documents for Human GLI-2 Biotinylated Antibody
Product Specific Notices for Human GLI-2 Biotinylated Antibody
For research use only
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