Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7248
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glucosaminyl (N-acetyl) Transferase 1/GCNT1
Arg33-His428
Accession # NP_001481
Arg33-His428
Accession # NP_001481
Specificity
Detects human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody
Detection of Human Glucosaminyl (N‑acetyl) Transferase 1/GCNT1 by Western Blot.
Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, TT human medullary thyroid cancer cell line, and Capan-1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Glucosaminyl (N-acetyl) Transferase 1/GCNT1 in HT‑29 human cell line.
Glucosaminyl (N-acetyl) Transferase 1/GCNT1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Glucosaminyl (N-acetyl) Transferase 1/GCNT1 in Human Bladder Cancer Tissue.
Glucosaminyl (N-acetyl) Transferase 1/GCNT1 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Sheep Anti-Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7248) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human bladder cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human bladder cancer tissue
Western Blot
1 µg/mL
Sample: SW480 human colorectal adenocarcinoma cell line, TT human medullary thyroid cancer cell line, and Capan‑1 human pancreatic adenocarcinoma cell line
Sample: SW480 human colorectal adenocarcinoma cell line, TT human medullary thyroid cancer cell line, and Capan‑1 human pancreatic adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glucosaminyl (N-acetyl) Transferase 1/GCNT1
Mucin-type O-glycans are initiated with an O-GalNAc attachment to a serine or threonine in a polypeptide. The O-GalNAc residues are subsequently extended by various glycosyltransferases resulting in different types of O-glycans. Most O-glycans contain the core 1 structure, Gal beta1-3GalNAc. Glucosaminyl (N-acetyl) Transferase 1 (GCNT1) converts the core 1 O-glycan to core 2 O-glycan, Gal beta1-3(GlcNAc beta1-6)GalNAc, via the addition of a GlcNAc residue (1, 2). Various ligand carbohydrates can be formed from core 2 branched oligosaccharides. For example, sialyl Lex in mucin‑type glycoproteins of blood cells can be formed from core 2 branched oligosaccharides (3, 4). The expression of GCNT1 was found to be associated with the progression of various types of cancer (5, 6, 7). The enzymatic activity of the recombinant GCNT1 is measured using a phosphatase-coupled method (8).
References
- Bierhuizen, M.F. (1993) Genes & Development 7:468.
- Yeh, J.C. et al. (1999) J. Biol. Chem. 274:3215.
- Hemmerich, S. et al. (1995) J. Biol. Chem. 270:12035.
- Wilkins, P.P. et al. (1996) J. Biol. Chem. 270:18732.
- Shimodaira, K. et al. (1997) Cancer Res. 57: 5201.
- Hatakyama, S. et al. (2010) Int. J. Cancer 127:1052.
- St Hill, C.A. et al. (2009) BMC Cancer 9:79.
- Wu, Z.L. et al. (2011) Glycobiology 21:727.
Long Name
Glucosaminyl (N-acetyl) Transferase 1 Core 2
Alternate Names
C2GNT-L, C2GNT1, Core 2 GNT, G6NT, NACGT2
Gene Symbol
GCNT1
UniProt
Additional Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Products
Product Documents for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody
Product Specific Notices for Human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody
For research use only
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