Human GluR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11333
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1059613
Product Specifications
Immunogen
Human GluR2/GRIA2 containing peptide
Accession # P42262
Accession # P42262
Specificity
Detects human GluR2/GRIA2 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GluR2 Antibody
Detection of GluR2 in TT (Positive) and THP-1 (Negative) cells.
GluR2 was detected in immersion fixed TT Human Medullary Thyroid Cancer Cells (Positive) and absent in THP-1 Human Acute Monocytic Leukemia Cells (Negative) using Mouse Anti-Human GluR2 Monoclonal Antibody (Catalog # MAB11333) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of GluR2 in Human Hippocampus.
GluR2 was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human GluR2 Monoclonal Antibody (Catalog # MAB11333) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human GluR2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed TT Human Medullary Thyroid Cancer Cell Line (Positive) and THP-1 Human Acute Monocytic Ieukemia Cell Line (Negative)
Sample: Immersion fixed TT Human Medullary Thyroid Cancer Cell Line (Positive) and THP-1 Human Acute Monocytic Ieukemia Cell Line (Negative)
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GluR2
References
- Entrez Gene: GRIA glutamate receptor, ionotropic, AMPA 2.
- Seeburg PH, Single F, Kuner T, Higuchi M, Sprengel R. "Genetic Manipulation of Key Determinants of Ion Flow in Glutamate Receptor Channels in the Mouse". Brain Res. 2001 Jul; 907(1-2):233.
- Cleveland DW, Rothstein JD. "From Charcot to Lou Gehrig: Deciphering Selective Motor Neuron Death in ALS". Nat. Rev. Neurosci. 2001 Nov; 2(11):806.
- Maas S, Patt S, Schrey M, Rich A. "Underediting of Glutamate Receptor GluR-B mRNA in Malignant Gliomas". Proc. Natl. Acad. Sci. U.S.A. 2001 Dec; 98(25):14687.
- Vivero M, Doyle L.A, Fletcher C.D., Mertens F, Hornick J.L. "GRIA2 is a Novel Diagnostic Marker for Solitary Fibrous Tumour Identified through Gene Expression Profiling". Histopathology. 2014. 65(1):71.
Long Name
Glutamate Receptor 2
Alternate Names
AMPA2, GluA2, GluR-K2, GLURB, GRIA2, HBGR2
Gene Symbol
GRIA2
UniProt
Additional GluR2 Products
Product Documents for Human GluR2 Antibody
Product Specific Notices for Human GluR2 Antibody
For research use only
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