Human Glutaminyl-peptide Cyclotransferase/QPCT Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6368

R&D Systems, part of Bio-Techne
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AF6368
AF6368-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Glutaminyl-peptide Cyclotransferase/QPCT Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Glutaminyl-peptide Cyclotransferase/QPCT
Ala33-Leu361
Accession # Q16769

Specificity

Detects human Glutaminyl-peptide Cyclotransferase/QPCT in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Glutaminyl-peptide Cyclotransferase/QPCT Antibody

Glutaminyl-peptide Cyclotransferase/QPCT antibody in Human Brain by Immunohistochemistry (IHC-P).

Glutaminyl-peptide Cyclotransferase/QPCT in Human Brain.

Glutaminyl-peptide Cyclotransferase/QPCT was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Glutaminyl-peptide Cyclotransferase/QPCT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6368) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Glutaminyl-peptide Cyclotransferase/QPCT Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutaminyl-peptide Cyclotransferase/QPCT

Glutaminyl-peptide Cyclotransferase, also known as Glutaminyl Cyclase, catalyzes the conversion of N‑terminal L-glutaminyl residues of peptides to pyroglutamyl groups (1). The enzyme is present in the pituitary and adrenal glands, where it is important for the generation of the N‑terminal pyroglutamyl groups of peptide hormones such as neurotensin and thyrotropin-releasing hormone. Glutaminyl Cyclase also catalyzes the conversion of N‑terminal L‑glutamyl residues to pyroglutamyl residues (2). This activity may contribute to the formation of several amyloid-related plaque forming peptides, contributing to Alzheimer’s disease pathology. Glutaminyl Cyclase is also considered to be a diagnostic marker of thyroid tumors (3).

References

  1. Busby, W.H. Jr. et al. (1987) J. Biol. Chem. 262:8532.
  2. Schilling, S. et al. (2004) FEBS Lett. 563:191.
  3. Griffith, O.L. et al. (2006) J. Clin. Oncol. 24:5043.

Alternate Names

GCT, Glutaminyl cyclase, Glutamyl cyclase

Entrez Gene IDs

25797 (Human)

Gene Symbol

QPCT

UniProt

Q16769

Additional Glutaminyl-peptide Cyclotransferase/QPCT Products

Product Documents for Human Glutaminyl-peptide Cyclotransferase/QPCT Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Glutaminyl-peptide Cyclotransferase/QPCT Antibody

For research use only

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