Human GPRASP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7078
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 698742
Product Specifications
Immunogen
E. coli-derived recombinant human GPRASP2
Gly47-Asp371
Accession # Q96D09
Gly47-Asp371
Accession # Q96D09
Specificity
Detects human GRASP2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GPRASP2 Antibody
Detection of Human GPRASP2 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPRASP2 Monoclonal Antibody (Catalog # MAB7078) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GPRASP2 at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GPRASP2 in Human Brain.
GPRASP2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human GPRASP2 Monoclonal Antibody (Catalog # MAB7078) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human GPRASP2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Western Blot
1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line
Sample: SH‑SY5Y human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPRASP2
Long Name
G Protein-coupled Receptor Associated Sorting Protein 2
Alternate Names
FLJ35662, FLJ37327, G protein-coupled receptor associated sorting protein 2, GASP2, GASP-2, G-protein coupled receptor-associated sorting protein 2
Gene Symbol
GPRASP2
UniProt
Additional GPRASP2 Products
Product Documents for Human GPRASP2 Antibody
Product Specific Notices for Human GPRASP2 Antibody
For research use only
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