Human HES-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3317
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 960216
Product Specifications
Immunogen
E. coli-derived recombinant human HES-1
Asn90-Pro277
Accession # Q14469
Asn90-Pro277
Accession # Q14469
Specificity
Detects human HES-1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human HES-1 Antibody
HES‑1 in MCF‑7 Human Cell Line.
HES-1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HES-1 Monoclonal Antibody (Catalog # MAB3317) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
HES‑1 in Human Liver Cancer Tissue.
HES-1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human HES-1 Monoclonal Antibody (Catalog # MAB3317) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human HES-1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue
Reviewed Applications
Read 1 review rated 5 using MAB3317 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HES-1
Long Name
Hairy And Enhancer of Split 1
Alternate Names
HES1, HL, HRY
Gene Symbol
HES1
UniProt
Additional HES-1 Products
Product Documents for Human HES-1 Antibody
Product Specific Notices for Human HES-1 Antibody
For research use only
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