Human Hexosaminidase A/HEXA Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6237

R&D Systems, part of Bio-Techne
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AF6237
AF6237-SP
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Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Hexosaminidase A/HEXA Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Hexosaminidase A/HEXA
Leu23-Thr529
Accession # P06865

Specificity

Detects human Hexosaminidase A/HEXA in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Hexosaminidase A/HEXA Antibody

Detection of Human Hexosaminidase A/ HEXA antibody by Western Blot.

Detection of Human Hexosaminidase A/ HEXA by Western Blot.

Western blot shows lysate of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Hexosaminidase A/HEXA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6237) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hexosaminidase A/HEXA at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Hexosaminidase A/HEXA antibody by Simple WesternTM.

Detection of Human Hexosaminidase A/HEXA by Simple WesternTM.

Simple Western lane view shows lysate of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Hexosaminidase A/HEXA at approximately 59 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Hexosaminidase A/HEXA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6237) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Hexosaminidase A/HEXA Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Hep3B human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hexosaminidase A/HEXA

HEXA (beta-hexosaminidase subunit alpha; also hexaminidase subunit alpha) is a 50-56 kDa glycoprotein member of the glycosyl hydrolase #20 family of enzymes. It is found in the lysosomes of a variety of cell types including fibroblasts, lacrimal acinar cells, and neurons. beta-Hexosaminidase is a multimeric enzyme that cleaves aminoacetylhexosamines from multiple glycosylated molecules. It exists in three noncovalent isozyme forms, among which is an A form that is trimeric and composed of one alpha- and two beta-subunits, a B form that is tetrameric and contains two alpha- and two beta-subunits, and a dimeric S form that contains two alpha-subunits. The alpha- and beta-subunits are products of related but distinct genes. The beta-hexosiminidase A form acts on a wide range of substrates, while the B form activity is biased towards sulfated hexoses. Human HEXA is synthesized as a 529 aa (amino acid) prepro-precursor. It contains a 22 aa signal sequence, a 66 aa prosegment, and a 441 aa mature region that contains a catalytic domain (aa 167-488). The prosegment may undergo additional processing through His90, and this segment (≈ 6 kDa) is known to remain linked to the mature region via a disulfide bond. Notably, circulating HEXA is comprised only of subunit proforms whose MW may run some 10 kDa higher in SDS-PAGE. Over aa 23-529, human HEXA shares 85% and 57% aa sequence identity with mouse HEXA and the human HEXA beta-subunit proprecursor, respectively.

Alternate Names

HEXA, TSD

Entrez Gene IDs

3073 (Human)

Gene Symbol

HEXA

UniProt

P06865

Additional Hexosaminidase A/HEXA Products

Product Documents for Human Hexosaminidase A/HEXA Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Hexosaminidase A/HEXA Antibody

For research use only

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