Human HGF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB294
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Porcine
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Bioassay, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry-Paraffin, Luminex Development, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 24612
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession # P14210
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession # P14210
Specificity
Detects human HGF in Western blots. In Western blots, no-cross reactivity with recombinant mouse HGF or recombinant canine HGF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human HGF Antibody
Detection of Recombinant Human, Mouse, and Canine HGF by Western Blot.
Western blot shows 25 ng of Recombinant Human HGF (Catalog # 294-HG), Recombinant Mouse HGF (2207-HG) and Recombinant Canine HGF (3386-HG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HGF at approximately 75 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
HGF in Human Lung Adenocarcinoma Tissue.
HGF was detected in immersion fixed paraffin-embedded sections of human lung adenocarcinoma tissue using Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Cell Proliferation Induced by HGF and Neutralization by Human HGF Antibody.
Recombinant Human HGF (Catalog # 294-HG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HGF (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294). The ND50 is typically 0.1-0.3 µg/mL.Applications for Human HGF Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Western Blot
1 µg/mL
Sample: Recombinant Human HGF (Catalog # 294-HG)
under non-reducing conditions only
Sample: Recombinant Human HGF (Catalog # 294-HG)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize HGF-induced proliferation in the 4MBr-5 rhesus monkey epithelial cell line. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 100 ng/mL Recombinant Human HGF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB294 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF
Long Name
Hepatocyte Growth Factor
Alternate Names
DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF
Entrez Gene IDs
Gene Symbol
HGF
UniProt
Additional HGF Products
Product Documents for Human HGF Antibody
Product Specific Notices for Human HGF Antibody
For research use only
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