Human HNF-6/ONECUT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6277
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HNF-6/ONECUT1
Gln139-Gly283
Accession # Q9UBC0
Gln139-Gly283
Accession # Q9UBC0
Specificity
Detects human HNF-6/ONECUT1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human ONECUT2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human HNF-6/ONECUT1 Antibody
Detection of Human HNF‑6/ONECUT1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for HNF-6/ONECUT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HNF‑6/ONECUT1 in HepG2 Human Cell Line.
HNF-6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of HNF-6/ONECUT1 in HepG2 (positive) and HDLM-2 (negative) cells.
HNF-6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cells (positive) and absent in HDLM-2 human Hodgkin’s lymphoma cells (negative) using Sheep Anti-Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human HNF-6/ONECUT1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and HDLM-2 human Hodgkin's lymphoma cells
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and HDLM-2 human Hodgkin's lymphoma cells
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HNF-6/ONECUT1
HNF-6 (also ONECUT1; one "cut" domain family member 1; OC-1) is a monomeric 53-56 kDa member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic hepatic and pancreatic islet anlage, plus adult hepatocytes and intestinal epithelial cells. HNF-6 is a transcriptional activator that is known to interact with Foxa2. Genes regulated by HNF-6 include Glut2, alpha1AT, alpha-fetoprotein and HNF-3 beta. Human HNF-6 is 465 amino acids (aa) in length. It contains two DNA-binding regions, one classified as a CUT domain (aa 283-369) and another as a homeobox domain (aa 385-444). HNF-6 is both acetylated and phosphorylated, contributing to protein stability and transactivation activity. Over aa 139-283, human HNF 6 shares 99% aa identity with mouse HNF-6. Rat HNF-6 shows a 26 aa insertion after Ala368. Given an overall aa identity of 99% between rat and human, the possibility exists that this insertion may also exist in human.
Long Name
Hepatocyte Nuclear Factor 6
Alternate Names
HNF-6A, HNF6, OC-1, ONECUT1
Gene Symbol
ONECUT1
UniProt
Additional HNF-6/ONECUT1 Products
Product Documents for Human HNF-6/ONECUT1 Antibody
Product Specific Notices for Human HNF-6/ONECUT1 Antibody
For research use only
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