Human HOXB13 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8156
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived human HOXB13
Met1-Gln102
Accession # Q92826
Met1-Gln102
Accession # Q92826
Specificity
Detects human HOXB13 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human HOXB13 Antibody
Detection of Human HOXB13 by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HOXB13 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HOXB13 in LNCaP Human Cell Line.
HOXB13 was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
HOXB13 in Human Prostate.
HOXB13 was detected in formalin fixed paraffin-embedded sections of human prostate using Sheep Anti-Human HOXB13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8156) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human HOXB13 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line
Sample: Immersion fixed LNCaP human prostate cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Western Blot
1 µg/mL
Sample: LNCaP human prostate cancer cell line
Sample: LNCaP human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HOXB13
HOXB13 (Homeobox B13) is a member of the homeobox family of transcription factors with a reported molecular weight of approximately 34 kDa. It is 284 amino acids in length and shares 93% aa identity with mouse and rat HOXB13. HOXB13 plays an important role in prostate cancer. Its expression promotes metastatis of prostate cancers and mutations in HOXB13 are associated with early onset disease. Elevated expression of HOXB13 is also associated with aggressive breast cancers, which may be due in part to its ability to reduce the expression of Estrogen Receptor alpha and increase expression of IL-6.
Long Name
Homeobox B13
Alternate Names
PSGD
Gene Symbol
HOXB13
UniProt
Additional HOXB13 Products
Product Documents for Human HOXB13 Antibody
Product Specific Notices for Human HOXB13 Antibody
For research use only
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