Human HTRA2/Omi Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1458
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 229926
Product Specifications
Immunogen
E. coli-derived recombinant human HTRA2/Omi
Ala134-Glu458
Accession # O43464
Ala134-Glu458
Accession # O43464
Specificity
Detects the mitochondria-processed form of human HTRA2/Omi.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human HTRA2/Omi Antibody
Detection of Human HTRA2/Omi by Western Blot.
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA2/Omi Monoclonal Antibody (Catalog # MAB1458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA2/Omi at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
HTRA2/Omi in K562 Human Cell Line.
HTRA2/Omi was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human HTRA2/Omi Monoclonal Antibody (Catalog # MAB1458) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human HTRA2/Omi Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Western Blot
1 µg/mL
Sample: SK-Mel-28 human malignant melanoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: SK-Mel-28 human malignant melanoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HTRA2/Omi
HTRA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HTRA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HTRA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (R&D Systems, Catalog # 786-XB) inhibition of Caspase-7 (R&D Systems, Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HTRA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site.
References
- Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
- van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
- Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
- Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
- Martins, L. et al. (2002) J. Biol. Chem. 277:439.
- Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
- Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.
Long Name
High Temperature Requirement Protein-2
Alternate Names
Omi, PRSS25
Gene Symbol
HTRA2
UniProt
Additional HTRA2/Omi Products
Product Documents for Human HTRA2/Omi Antibody
Product Specific Notices for Human HTRA2/Omi Antibody
For research use only
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