Human ICAM-3/CD50 Antibody
R&D Systems, part of Bio-Techne | Catalog # BBA15
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Cited:
Adhesion Assay, Direct ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # ICAM-3.3
Product Specifications
Immunogen
Recombinant human ICAM-3 Fc Chimera
Specificity
Detects human ICAM‑3/CD50 in ELISAs and Western blots. ICAM‑3/CD50 was found not to recognize COS cells expressing the humanICAM‑1 protein. The epitope for ICAM‑3/CD50 has been found to be located on domain 1, or the interface between domains 1 and 2 of the human ICAM‑3/CD50 molecule.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human ICAM-3/CD50 Antibody
ICAM‑3/CD50 in Human PBMCs.
ICAM-3/CD50 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # BBA15) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human ICAM-3/CD50 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Immunocytochemistry
Cattoretti, G. et al. (1992) J. Pathol. 168:357.
Immunoprecipitation
Vazeux, R. et al. (1992) Nature 360:485.
Western Blot
1 µg/mL
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Human ICAM-3/CD50 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-3/CD50
Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alphad/ beta2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.
References
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Entrez Gene IDs
3385 (Human)
Gene Symbol
ICAM3
Additional ICAM-3/CD50 Products
Product Documents for Human ICAM-3/CD50 Antibody
Product Specific Notices for Human ICAM-3/CD50 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...