Human IFN-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8142
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2036A
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IFN‑ beta
Met1-Asn187
Accession # P01574
Met1-Asn187
Accession # P01574
Specificity
Detects human IFN‑ beta in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human IFN-beta Antibody
IFN‑ beta in A549 Human Cell Line.
IFN-beta was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human IFN-beta Monoclonal Antibody (Catalog # MAB8142) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of IFN-beta in M1 macrophages by Flow Cytometry.
M1 macrophages treated with 50 ng/mL each of rhGM-CSF (215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (285-IF) and restimulated with monensin for 3 hours (3uM) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Rabbit Anti-Human IFN-beta Monoclonal Antibody (Catalog # MAB8142) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Intracellular Molecules.Applications for Human IFN-beta Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µL/106 cells
Sample: M1 macrophages treated with 50 ng/mL each of rhGM-CSF (Catalog # 215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (Catalog # 285-IF) and restimulated with monensin for 3 hours (3uM)
Sample: M1 macrophages treated with 50 ng/mL each of rhGM-CSF (Catalog # 215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (Catalog # 285-IF) and restimulated with monensin for 3 hours (3uM)
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-beta
IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
UniProt
Additional IFN-beta Products
Product Documents for Human IFN-beta Antibody
Product Specific Notices for Human IFN-beta Antibody
*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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