Human IFN-epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB91472
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 995728
Product Specifications
Immunogen
E. coli-derived recombinant human IFN-epsilon
Leu22-Arg208
Accession # Q86WN2
Leu22-Arg208
Accession # Q86WN2
Specificity
Detects human IFN-epsilon in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IFN-epsilon Antibody
IFN-epsilon in HeLa Human Cell Line.
IFN-epsilon was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human IFN-epsilon Monoclonal Antibody (Catalog # MAB91472) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human IFN-epsilon Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-epsilon
Interferon-epsilon (IFNe) is a type I interferon with expression limited to the brain, lung, intestine and reproductive tract. It has been proposed that unlike other type I interferons, IFNe plays a role in mucosal immunity and protects against pathogens including chlamidia, HIV and herpes simplex virus 2. Its expression is upregulated by TNF alpha. Unlike other type-1 interferons, IFNe is also hormonally regulated, and mouse and human IFNe have been shown to have a binding site in their promoters for progesterone receptor. Epithelial cells isolated from uterine endometrium have higher IFNee expression in the proliferative phase, when estrogen levels are highest. IFNe is the only IFN isoform constituitively expressed in HeLa cells.
Long Name
Interferon epsilon
Alternate Names
Ifn-tau-11, IFNE, IFNepsilon, IFNT1, INFE1
Gene Symbol
IFNE
UniProt
Additional IFN-epsilon Products
Product Documents for Human IFN-epsilon Antibody
Product Specific Notices for Human IFN-epsilon Antibody
For research use only
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