Human IKK beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4535
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IKK beta
Gly374-Ser756
Accession # O14920
Gly374-Ser756
Accession # O14920
Specificity
Detects endogenous human IKK beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IKK alpha, IKK gamma, or IKK epsilon.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human IKK beta Antibody
Detection of Human IKK beta by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human IKK beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4535) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IKK beta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF4535 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK beta
I kappaB kinase beta (IKK beta) is also known as IKBKB and IKK2. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF-kappa B. Like IKK alpha, IKK beta contains kinase, leucine zipper, and helix‑loop‑helix domains. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha/IKK beta heterodimers.
Long Name
IkB Kinase beta
Alternate Names
IkBKB, IKK2, NFKBIKB
Gene Symbol
IKBKB
UniProt
Additional IKK beta Products
Product Documents for Human IKK beta Antibody
Product Specific Notices for Human IKK beta Antibody
For research use only
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