Human IL-13 R alpha2 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB614A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

CAR-T (Flow Cytometry)

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

View all IL-13 R alpha 2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-13 R alpha2
Cys22-Leu342
Accession # Q14627

Specificity

Detects human IL-13 R alpha2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-13 R alpha2 APC-conjugated Antibody

Detection of IL-13 Ra2 antibody in A375 Human Cell Line antibody by Flow Cytometry.

Detection of IL‑13 R alpha2 in A375 Human Cell Line by Flow Cytometry.

A375 human melanoma cell line was stained with Goat Anti-Human IL-13 Ra2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB614A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-13 R alpha2 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: A375 human melanoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-13 R alpha 2

Interleukin-13 Receptor alpha 2 (IL-13 R alpha2), also CD213 alpha2, is a 55-57 kDa member of the type I cytokine receptor family of molecules. Human IL-13 R alpha2 is a 354 amino acid (aa) type I transmembrane glycoprotein that contains a 317 aa extracellular domain (ECD) characterized by three fibronectin type III domains (aa 27-343). It is expressed by a limited number of cell types, principally those associated with the respiratory system. These cells include fibroblasts, type II alveolar and visceral smooth muscle cells, pseudostratified ciliated epithelium, goblet cells and possibly monocytes. Notably, IL-13 R alpha2 is often found to be intracellular, rather than plasma membrane expressed, and this may reflect storage deposits that can be quickly accessed when needed. IL-13 R alpha2 is one of two IL-13Rs that bind IL-13. The principal, but low affinity receptor for IL-13 is a 65 kDa transmembrane protein termed IL-13 R alpha1. Following binding to IL-13, this protein complexes with an adjacent IL-4 R alpha receptor on the cell surface to form a high affinity, functional receptor signaling complex for IL-13. Within this system, IL-13 R alpha2 has been described as a "decoy receptor" that binds to circulating IL-13, rendering it unavailable for IL-13 R alpha1 binding. Supporting this view is the fact that there does not appear to be any canonical signaling motif in IL-13 R alpha2s short cytoplasmic tail, and the fact that soluble IL-13 R alpha2 will bind to IL-13 and neutralize its effects on target cells in culture. The latter point is of particular importance when it is recognized that IL-13 R alpha2 has a naturally circulating soluble splice form in mouse. Studies in human, however, suggest a more complicated situation. First, it is unclear if human actually has circulating IL-13 R alpha2. Unlike in mouse, there is no potential alternative splice form in human that could generate a soluble isoform. And while MMP-8 has been identified as a potential mediator of IL-13 R alpha2 proteolytic cleavage, such activity would appear to generate rather small quantities of soluble IL-13 R alpha2. As a transmembrane protein, IL-13 R alpha2 is suggested to interact with IL-4 R alpha, and this likely will result in a downregulation of IL-13 activity. Thus, IL-13 R alpha2, while not a decoy receptor, could act as a negative modulator of IL-13 signaling. On the other hand, the cytoplasmic tail of IL-13 R alpha2 is now believed to stimulate AP-1 activity, and the IL-13:IL-13 R alpha2 transmembrane complex has been identified as a component of a larger signaling receptor complex for the GH-18 (Glycosyl Hydrolase 18) family member YKL40/Chi3L1. As such, IL-13 R alpha2 is likely to exhibit multiple actions in a system and species-specific manner. Over aa 27-342, human and mouse IL-13 R alpha2 share 64% aa sequence identity.

Long Name

Interleukin 13 Receptor alpha 2

Alternate Names

CD213a2, IL-13Ra2, IL13R alpha 2, IL13RA2

Entrez Gene IDs

3598 (Human); 16165 (Mouse)

Gene Symbol

IL13RA2

UniProt

Q14627

Additional IL-13 R alpha 2 Products

Product Documents for Human IL-13 R alpha2 APC-conjugated Antibody

Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-13 R alpha2 APC-conjugated Antibody

For research use only

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