Human IL-17F Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB13351
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 197301
Product Specifications
Immunogen
E. coli-derived recombinant human IL-17F
Arg31-Gln163
Accession # Q96PD4
Arg31-Gln163
Accession # Q96PD4
Specificity
Detects human IL-17F in direct ELISAs. Does not cross-react with recombinant human IL‑17A, B, C, D, or E.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IL-17F Antibody
Detection of IL‑17F in Activated Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours (dark orange filled histogram), then stained with Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13351) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
IL‑17F in Human Th17 Cells.
IL-17F was detected in immersion fixed human T helper 17 cells using Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13351) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human IL-17F Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human T helper 17 cells
Sample: Immersion fixed human T helper 17 cells
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with PMA and calcium ionomycin, fixed with paraformaldehyde and permeabilized with saponin
Sample: Human peripheral blood mononuclear cells treated with PMA and calcium ionomycin, fixed with paraformaldehyde and permeabilized with saponin
Reviewed Applications
Read 1 review rated 5 using MAB13351 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17F
Long Name
Interleukin 17F
Alternate Names
IL17F
Gene Symbol
IL17F
UniProt
Additional IL-17F Products
Product Documents for Human IL-17F Antibody
Product Specific Notices for Human IL-17F Antibody
For research use only
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