Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-17RA/IL-17R Antibody

Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). View our protocol for Staining Membrane-associated Proteins.

CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody.

Recombinant Human IL-17/IL-17A induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GROa DuoSet (DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-17/IL-17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177). The ND₅₀ is typically 0.4-2 µg/mL.

IL-17 RA/IL-17 R Specificity is Shown by Flow Cytometry in Knockout Cell Line.

IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-17RA/IL-17R Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: K562 human chronic myelogenous leukemia cell line

Knockout Validated

IL-17 RA/IL-17 R is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in IL-17 RA/IL-17 R knockout K562 cell line.

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)

Neutralization

Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

References

Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Long Name

Interleukin 17 Receptor

Alternate Names

CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Gene Symbol

IL17RA

UniProt

Q96F46

Additional IL-17RA/IL-17R Products

Product Documents for Human IL-17RA/IL-17R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-17RA/IL-17R Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-17RA/IL-17R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB177

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications