Human IL-17RA/IL-17R APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB177A
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Knockout Validated
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 133617
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-17 R
Leu33-Trp320
Accession # Q96F46
Leu33-Trp320
Accession # Q96F46
Specificity
Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-17RA/IL-17R APC-conjugated Antibody
Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R APC-conjugated Monoclonal Antibody (Catalog # FAB177A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
IL-17 RA/IL-17 R Specificity is Shown by Flow Cytometry in Knockout Cell Line.
IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R APC-conjugated Monoclonal Antibody (Catalog # FAB177A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.Applications for Human IL-17RA/IL-17R APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Knockout Validated
IL-17 RA/IL-17 R is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in IL-17 RA/IL-17 R knockout K562 cell line.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17RA/IL-17R
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
References
- Yao, Z. et al. (1997) Cytokine 9:794.
- Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Long Name
Interleukin 17 Receptor
Alternate Names
CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA
Entrez Gene IDs
Gene Symbol
IL17RA
UniProt
Additional IL-17RA/IL-17R Products
Product Documents for Human IL-17RA/IL-17R APC-conjugated Antibody
Product Specific Notices for Human IL-17RA/IL-17R APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...