Human IL-1 beta/IL-1F2 APC-conjugated Antibody

Detection of IL‑1 beta/IL‑1F2 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes either (A) untreated or (B) treated with 250 ng/mL LPS overnight were stained with Rabbit Anti-Human IL-1 beta/IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC8406A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC105A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human IL-1 beta/IL-1F2 by Flow Cytometry

Knock-down of ATG4D by shRNA in UTSM cell line mimic UF phenotype. (a) Autophagy markers analysis by FACS with intracellular staining and mean fluorescence intensity MFI for LC3, and (b) by western blot for LC3 and P62. (c) Extracellular matrix markers analysis by western blot. (d) Endogenous quantification of pro-inflammatory cytokines expression was done by intracellular staining for TNF-alpha, IL-1 beta, TGF-beta and IL-10 using flow cytometry analysis. The expression level was represented as mean fluorescence intensity (MFI). Data from the histogram are shown as mean±S.D. and are representative of three independent experiments. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddiscovery201741), licensed under a CC-BY license. Not internally tested by R&D Systems.