Human IL-34 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5265
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
In vivo assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-34
Asn21-Pro242
Accession # NP_689669
Asn21-Pro242
Accession # NP_689669
Specificity
Detects human IL-34 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse IL-34 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-34 Antibody
Cell Proliferation Induced by IL-34 and Neutralization by Human IL-34 Antibody.
Recombinant Human IL-34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265). The ND50 is typically 0.035-0.175 µg/mL.
Detection of Human IL-34 by Western Blot.
Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-34 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IL-34 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human brain cortex tissue
Sample: Human brain cortex tissue
Neutralization
Measured by its ability to neutralize IL‑34-induced proliferation in human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The Neutralization Dose (ND50) is typically 0.035-0.175 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑34.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-34
Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).
References
- Lin, H. et al. (2008) Science 320:807.
Long Name
Interleukin 34
Alternate Names
C16orf77, IL34
Gene Symbol
IL34
UniProt
Additional IL-34 Products
Product Documents for Human IL-34 Antibody
Product Specific Notices for Human IL-34 Antibody
For research use only
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