Human IL-6R alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB227R
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 17506R
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R alpha
Leu20-Asp339
Accession # P08887
Leu20-Asp339
Accession # P08887
Specificity
Detects human IL-6 R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human(rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-6R alpha Antibody
Detection of IL‑6 R alpha in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Human IL-6 R alpha Monoclonal Antibody (Catalog # MAB227R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgGF(ab')2 Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
IL-6 R alpha Enhancement of IL-6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6 R alpha Antibody.
Recombinant Human IL-6 Ra (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6 Ra Monoclonal Antibody (Catalog # MAB227R). The ND50 is typically 0.04-0.2 µg/mL.Applications for Human IL-6R alpha Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: U937 Human Cell Line
Sample: U937 Human Cell Line
Western Blot
1 µg/mL
Sample: Recombinant Human IL‑6 R alpha (Catalog # 227-SR)
Sample: Recombinant Human IL‑6 R alpha (Catalog # 227-SR)
Neutralization
Measured by its ability to neutralize IL‑6 R alpha-mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.
Human IL-6R alpha Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6R alpha
The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).
A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alphas have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).
References
- Yamasaki et al. (1988) Science 241:825.
- Baumann et al. (1990) J. Biol. Chem. 265:19853.
- Hibi et al. (1990) Cell 63:1149.
- Schooltink et al. (1991) Eur. J. Biochem. 277:659.
- Novick et al. (1989) J. Exp. Med. 170:1409.
- Yasukawa et al. (1990) J. Biochem. 108:673.
- Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Alternate Names
CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha
Gene Symbol
IL6R
UniProt
Additional IL-6R alpha Products
Product Documents for Human IL-6R alpha Antibody
Product Specific Notices for Human IL-6R alpha Antibody
For research use only
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