Detects human CXCL8/IL‑8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL‑8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IL-8/CXCL8 Fluorescein-conjugated Antibody

Detection of CXCL8/IL-8 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL-8 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC208F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P), as shown in panel A. Inhibition of IC208F staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human IL-8/CXCL8 Fluorescein-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood monocytes treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-8/CXCL8

CXCL8/IL-8 is an 8-9 kDa member of the CXCL subfamily of chemokines. It was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It has also referred to as Neutrophil Activating Protein (NAP), Monocyte-derived Neutrophil Chemotactic Factor (MDNCF) and granulocyte chemotactic protein (GCP). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and mammary plus alveolar epithelium can produce CXCL8 in response to a wide variety of stimuli, such as IL-1b, TNF-a, LPS, MRP-8/14 and viruses. When secreted, it circulates as both a monomer and dimer, with the dimeric forms being a homodimer and heterodimer with CXCL4/PF4. CXCL8 binds to CXCR-1 and -2, with the CXCL8 monomer favoring CXCR-1, and the CXCL8 homodimer favoring CXCR-2 and glycosaminoglycans. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. It also induces expression of the cell adhesion molecules CD11/CD18, and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes, and has also shown to be an autocrine growth factor for melanoma cells. CXCL8 is also reported to be angiogenic both in vivo and in vitro. There is no direct structural rodent counterpart to human IL-8.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Gene Symbol

CXCL8

UniProt

P10145

Additional IL-8/CXCL8 Products

Product Documents for Human IL-8/CXCL8 Fluorescein-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human IL-8/CXCL8 Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human IL-8/CXCL8 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC208F

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications