Human IL-8/CXCL8 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # MAB208
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Bacteria - Escherichia coli, Bacteria - Porphyromonas gingivalis, Bovine, Complex Species Category, Guinea Pig, Rabbit
Applications
Validated:
CyTOF-ready, ELISA Capture (Matched Antibody Pair), Immunocytochemistry, Intracellular Staining by Flow Cytometry, Neutralization, Simple Western, Western Blot
Cited:
Affinity Chromatography, Antibody Array Development, Array Development, Binding Assay, Bioassay, Blocking, Chromatin Immunoprecipitation (ChIP), Co-Immunoprecipitation, Electrochemiluminescent Assay, ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Inhibition, Luminex Development, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6217
Product Specifications
Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Ser28-Ser99
Accession # P10145
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-8/CXCL8 Antibody
Detection of Human IL-8/CXCL8 by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
IL-8/CXCL8 in Human PBMCs.
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human IL-8/CXCL8 by Simple WesternTM.
Simple Western lane view shows human PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.Applications for Human IL-8/CXCL8 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Sample: Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Simple Western
50 µg/mL
Sample: Human PBMC conditioned media treated with LPS
Sample: Human PBMC conditioned media treated with LPS
Western Blot
3 µg/mL
Sample: THP-1 human acute monocytic leukemia cell line treated (+) with PMA and LPS
Sample: THP-1 human acute monocytic leukemia cell line treated (+) with PMA and LPS
Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.
Human IL-8/CXCL8 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 11 reviews rated 4.8 using MAB208 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8
CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.
Long Name
Interleukin 8
Alternate Names
CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1
Gene Symbol
CXCL8
UniProt
Additional IL-8/CXCL8 Products
Product Documents for Human IL-8/CXCL8 Antibody
Product Specific Notices for Human IL-8/CXCL8 Antibody
For research use only
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