Human Importin  alpha2/KPNA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB62071

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB62071
MAB62071-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 682208

View all Importin alpha 2/KPNA2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Importin alpha2/KPNA2
Ser2-Asn132
Accession # P52292

Specificity

Detects human Importin alpha2/KPNA2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) KPNA1, rhKPNA3, rhKPNA4, rhKPNA5, or rhKPNB1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Importin  alpha2/KPNA2 Antibody

Importin a2/KPNA2 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

Importin alpha2/KPNA2 in NTera‑2 Human Cell Line.

Importin a2/KPNA2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB62071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Importin  alpha2/KPNA2 in HeLa Human Cell Line.

Importin  alpha2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin  alpha2/KPNA2 Monoclonal Antibody (Catalog # MAB62071) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Importin  alpha2/KPNA2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 2/KPNA2

Importin subunit alpha2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, and RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha2 first binds to NBS1, and then to Importin beta1, which mediates movement through the nuclear pore. Human Importin alpha2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin b domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. The aminoacid region 1-132 of human Importin alpha2 shares a 94% homology with mouse Importin alpha2.

Long Name

Karyopherin alpha 2

Alternate Names

Importin alpha 1, IPOA1, KPNA2, Pendulin, QIP2, RCH1, SRP1-alpha

Entrez Gene IDs

3838 (Human); 16647 (Mouse); 85245 (Rat)

Gene Symbol

KPNA2

UniProt

P52292 (Human)

Additional Importin alpha 2/KPNA2 Products

Product Documents for Human Importin  alpha2/KPNA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Importin  alpha2/KPNA2 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.