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Human Integrin alpha 1/CD49a Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB56761G

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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FAB56761G-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rabbit IgG Clone # 1090A

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO derived recombinant human Integrin alpha1 beta1
Met1-Pro1141 (Integrin alpha1) & Gln21-Asp728 (Integrin beta1)
Accession # P56199

Specificity

Detects Integrin alpha1/CD49a in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human Integrin alpha 1/CD49a Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Integrin alpha 1/CD49a

Integrin alpha1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules.  It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium.  Integrin alpha1 forms a noncovalent heterodimer with Integrin beta1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI.  It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus.  Mature human Integrin alpha1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail.  The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites.  Potential splice variants exist that show a two and four aa substitution for aa 765-1179.  Over aa 29-1141 (the ECD), human Integrin alpha1 shares 88% aa identity with mouse Integrin alpha1. 

Alternate Names

CD49a, ITGA1, VLA-1

Entrez Gene IDs

3672 (Human); 109700 (Mouse); 25118 (Rat)

Gene Symbol

ITGA1

UniProt

Additional Integrin alpha 1/CD49a Products

Product Documents for Human Integrin alpha 1/CD49a Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin alpha 1/CD49a Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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