Human Integrin alpha1/CD49a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB56761
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1090A
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO derived recombinant human Integrin alpha1 beta1
Met1-Pro1141 (Integrin alpha1) & Gln21-Asp728 (Integrin beta1)
Accession # P56199
Met1-Pro1141 (Integrin alpha1) & Gln21-Asp728 (Integrin beta1)
Accession # P56199
Specificity
Detects Integrin alpha1/CD49a in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Integrin alpha1/CD49a Antibody
Detection of Integrin alpha1/CD49a in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human Integrin a1/CD49a Monoclonal Antibody (Catalog # MAB56761, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).Applications for Human Integrin alpha1/CD49a Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 1/CD49a
Integrin alpha1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin alpha1 forms a noncovalent heterodimer with Integrin beta1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765-1179. Over aa 29-1141 (the ECD), human Integrin alpha1 shares 88% aa identity with mouse Integrin alpha1.
Alternate Names
CD49a, ITGA1, VLA-1
Gene Symbol
ITGA1
UniProt
Additional Integrin alpha 1/CD49a Products
Product Documents for Human Integrin alpha1/CD49a Antibody
Product Specific Notices for Human Integrin alpha1/CD49a Antibody
For research use only
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