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Human Integrin  alpha4 beta7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10078

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

CyTOF-ready, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu117

Product Specifications

Immunogen

Human Integrin alpha4 beta7/LPAM‑1

Specificity

Detects human Integrin alpha4 beta7/LPAM‑1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Vedolizumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images

Detection of Integrin a4 beta7/LPAM-1 antibody in Human PBMC antibody by Flow Cytometry.

Detection of Integrin alpha4 beta7/LPAM‑1 in Human PBMC by Flow Cytometry.

Human PBMC were stained with (A) Human Anti-Human Integrin a4 beta7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10078) or (B) no primary antibody, followed by anti-Human IgG APC-conjugated Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC

Reviewed Applications

Read 1 review rated 4 using MAB10078 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4 beta 7/LPAM-1

Vedolizumab is a biosimilar that binds and blocks binds to integrin alpha4 beta7. This product can be used in flow cytometry or various assay formats to block or measure integrin alpha4 beta7.

Alternate Names

CD49d/beta7, LPAM-1, LPAM1

Entrez Gene IDs

3676 (Human); 16402 (Mouse)

Gene Symbol

ITGA4

Additional Integrin alpha 4 beta 7/LPAM-1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices

For research use only

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