HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 Monoclonal Antibody (Catalog # MAB1219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).

Integrin alphaV/CD51 in HT1080 Human Cell Line.

Integrin aV/CD51 was detected in immersion fixed HT1080 human fibrosarcoma cell line using 10 µg/mL Mouse Anti-Human Integrin aV/CD51 Monoclonal Antibody (Catalog # MAB1219) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin alphaV/CD51 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HT1080 human fibrosarcoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT1080 human fibrosarcoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB1219 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alphaV (CD51), also known as Vitronectin Receptor Subunit alpha is a 140‑150 kDa member of the integrin alpha-chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta-chains, including beta1, 3, 5, 6 and 8. Human alphaV is a 1018 amino acid (aa) type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta‑propellor domain (aa 46-483). Furin cleavage of the alphaV ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alphaV chain. alphaV-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2 and TSP. The ECD of human alphaV is 92% aa identical to mouse alphaV ECD.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Entrez Gene IDs

3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)

Gene Symbol

ITGAV

Additional Integrin alpha V/CD51 Products

Product Documents for Human Integrin alphaV/CD51 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Integrin alphaV/CD51 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alphaV/CD51 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1219

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Integrin alpha V/CD51 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alphaV/CD51 Antibody

Detection of Integrin alphaV/CD51 in HT1080 Human Cell Line by Flow Cytometry.