Human Integrin beta1/CD29 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1778
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4B7R
Product Specifications
Immunogen
Ocular melanoma cell line V+B2
Specificity
Detects human Integrin beta1/CD29 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Integrin beta1/CD29 Antibody
Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB1778, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).
Integrin beta1/CD29 in MG‑63 Human Cell Line.
Integrin beta1/CD29 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human Integrin beta1/CD29 Monoclonal Anti-body (Catalog # MAB1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Anti-body (yellow; NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Integrin beta1/CD29 in A549 Cells.
Integrin beta1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB1778) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.Applications for Human Integrin beta1/CD29 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells (PBMCs)
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line, A549 human lung carcinoma cell line
Sample: Immersion fixed MG-63 human osteosarcoma cell line, A549 human lung carcinoma cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human skin cancer tissue
Immunoprecipitation
Marshall, J.F. et al. (1998) Br. J. Cancer 77:522.
Western Blot
1 µg/mL
Sample: Recombinant Human Integrin beta1/CD29 under non-reducing conditions only
Sample: Recombinant Human Integrin beta1/CD29 under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
References
- Wayner, E.A. and W.G. Carter (1987) J. Cell Biol. 105:1873.
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
Additional Integrin beta 1/CD29 Products
Product Documents for Human Integrin beta1/CD29 Antibody
Product Specific Notices for Human Integrin beta1/CD29 Antibody
For research use only
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